Contact an Account ManagerDetermination of anticonvulsant drugs and methyl xanthine derivatives in serum
Summary
Type: HPLC
Application #: 103830
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV 230nm
Conditions: eluent A for precolumn loading and washing: 14mM NaH2PO4 - 6mM Na2HPO4,
eluent B for precolumn backflush and analytical separation: eluent A - acetonitrile - methanol (6.5:1.5:2, v/v),
flow rate 0.8ml/min; detection: UV, 230nm
Authors: Haginaka, J. et al.
Source: J. Chromatogr. 529 (1990) 455–461
Details
Legend: Chromatogram after pretreatment of serum with the home-made ISPR precolumn,
injection volume 20µl.
primidone (10µg/ml),
phenobarbital (10µg/ml,
phenytoin (20µg/ml),
carbamazepine (5µg/ml).